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4 结论 改良离子交换色谱法能迅速有效的检测人外周血白细胞全基因组DNA甲基化水平，糖尿病患者基因组DNA甲基化水平与健康对照无显著差异。 致谢 170 感谢中南大学湘雅二医院肾脏病研究所李峥医师对本文实验方法给予的技术指导和帮 助。 [参考文献] (References) [1] KDOQI. KDOQI Clinical Practice Guidelines and Clinical Practice Recommendations for Diabetes and 175 Chronic Kidney Disease[J]. Am J Kidney Dis, 2007, 49 (2 Suppl 2): S12-S154. [2] 胡善联, 刘国恩, 许樟荣, 等. 我国糖尿病流行病学和疾病经济负担研究现状[J]. 中国卫生经济, 2008, 27(8): 5-8. [3] Dwivedi RS, Herman JG, McCaffrey TA, Raj DS. Beyond genetics: epigenetic code in chronic kidney disease[J]. Kidney Int, 2011, 79(1): 23-32. 180 [4] Bechtel W, McGoohan S, Zeisberg EM, Müller GA, Kalbacher H, Salant DJ, Müller CA, Kalluri R, Zeisberg M. Methylation determines fibroblast activation and fibrogenesis in the kidney[J]. Nat Med, 2010, 16(5): 544-550. [5] 周宏, 易斌, 张浩. DNA甲基化修饰与肾脏疾病[J]. 中华肾脏病杂志, 2009, 25(8): 659-661. [6] Maier S, Olek A. Diabetes: a candidate disease for efficient DNA methylation profiling[J]. J Nutr, 2002, 132(8 Suppl): 2440S-2443S. 185 [7] Ramsahoye BH. Measurement of genome wide DNA methylation by reversed-phase high-performance liquid chromatography[J]. Methods, 2002, 27(2): 156-161. [8] Rozhon W, Baubec T, Mayerhofer J, et al. Rapid quantification of global DNA methylation by isocratic cation exchange high-performance liquid chromatography[J]. Anal Biochem, 2008, 375(2): 354-360. [9] 李峥, 李瑛, 杨永佳, 等. 离子交换色谱法检测人血白细胞基因组DNA整体甲基化水平[J]. 中国医师杂190 志, 2010, 12(1): 55-58. [10] K C Kuo, R A McCune, C W Gehrke, et al. Quantitative reversed-phase high performance liquid chromatographic determination of major and modified deoxyribonucleosides in DNA[J]. Nucleic Acids Res, 1980, 8(20): 4763-4776. [11] Dabelea D, Hanson RL, Lindsay RS, et al. Intrauterine exposure to diabetes conveys risks for type 2 diabetes 195 and obesity[J]. Diabetes, 2000, 49(12): 2208-2211. [12] Varrault A, Bilanges B, Mackay DJ, et al. Characterization of the methylation-sensitive promoter of the imprinted ZAC gene supports its role in transient neonatal diabetes mellitus[J]. J Biol Chem, 2001, 276(22): 18653-18656. [13] Laborie LB, Mackay DJ, Temple IK, et al. DNA hypomethylation, transient neonatal diabetes, and prune 200 belly sequence in one of two identical twins[J]. Eur J Pediatr, 2010, 169(2): 207-213. [14] Kuroda A, Rauch TA, Todorov I, et al. Insulin gene expression is regulated by DNA methylation[J]. PLoS One, 2009, 4(9): e6953. [15] Yang BT, Dayeh TA, Kirkpatrick CL, et al. Insulin promoter DNA methylation correlates negatively with insulin gene expression and positively with HbA(1c) levels in human pancreatic islets[J]. Diabetologia, 2011, 205 54(2): 360-367. [16] 王萍, 闫芳, 何忠效, 等. 糖尿病大鼠肾、胰基因组DNA甲基化状态的变化[J]. 北京师范大学学报, 2001, 37(2): 246-249. [17] Nanayakkara PW, Kiefte-de Jong JC, Stehouwer CD, et al. Association between global leukocyte DNA methylation, renal function, carotid intima-media thickness and plasma homocysteine in patients with stage 2-4 210 chronic kidney disease. Nephrol Dial Transplant, 2008, 23: 2586-2592. 原创学术论文网Tag：代写论文 论文发表 代写职称论文 代写医学论文 |
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